|Application ||WB, E|
|Other Accession||P97390, NP_009190.2|
|Calculated MW||65077 Da|
|Antigen Region||461-489 aa|
|Other Names||Vacuolar protein sorting-associated protein 45, h-VPS45, hlVps45, VPS45, VPS45A, VPS45B|
|Target/Specificity||This VPS45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 461-489 amino acids from the C-terminal region of human VPS45.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||VPS45 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.|
|Cellular Location||Golgi apparatus membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein Note=Associated with Golgi/endosomal vesicles and the trans-Golgi network.|
|Tissue Location||Ubiquitous. Expression was highest in testis, heart and brain, intermediate in kidney, spleen, prostate, ovary, small intestine and thymus and low in lung, skeletal muscle, placenta, colon, pancreas, peripheral blood leukocytes and liver|
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Provided below are standard protocols that you may find useful for product applications.
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. [provided by RefSeq].
Rahajeng, J., et al. Exp. Cell Res. 316(5):859-874(2010)
Kim, B.Y., et al. Biochem. Biophys. Res. Commun. 311(3):577-582(2003)
Dulubova, I., et al. EMBO J. 21(14):3620-3631(2002)
de Renzis, S., et al. Nat. Cell Biol. 4(2):124-133(2002)
Nielsen, E., et al. J. Cell Biol. 151(3):601-612(2000)
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