|Application ||WB, E|
|Other Accession||NP_647603.1, NP_005672.1|
|Calculated MW||52676 Da|
|Antigen Region||68-97 aa|
|Other Names||TATA box-binding protein-associated factor RNA polymerase I subunit A, RNA polymerase I-specific TBP-associated factor 48 kDa, TAFI48, TATA box-binding protein-associated factor 1A, TBP-associated factor 1A, Transcription factor SL1, Transcription initiation factor SL1/TIF-IB subunit A, TAF1A|
|Target/Specificity||This TAF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the N-terminal region of human TAF1A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TAF1A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre- initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits.|
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Provided below are standard protocols that you may find useful for product applications.
Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.
Greco-Stewart, V.S., et al. Virology 386(1):12-15(2009)
Yamamoto, K., et al. Mol. Cell. Biol. 24(14):6338-6349(2004)
Dynes, J.L., et al. J. Biochem. 135(3):429-438(2004)
Hirschler-Laszkiewicz, I., et al. J. Biol. Chem. 278(21):18953-18959(2003)
Yuan, X., et al. EMBO Rep. 3(11):1082-1087(2002)
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