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TAF1A Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TAF1A Antibody (N-term) AP16145A
    TAF1A Antibody (N-term) (Cat. #AP16145a) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the TAF1A antibody detected the TAF1A protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15573
Other Accession NP_647603.1, NP_005672.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52676 Da
Antigen Region 68-97 aa
Additional Information
Gene ID 9015
Other Names TATA box-binding protein-associated factor RNA polymerase I subunit A, RNA polymerase I-specific TBP-associated factor 48 kDa, TAFI48, TATA box-binding protein-associated factor 1A, TBP-associated factor 1A, Transcription factor SL1, Transcription initiation factor SL1/TIF-IB subunit A, TAF1A
Target/Specificity This TAF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the N-terminal region of human TAF1A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTAF1A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TAF1A
Function Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre- initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits.
Cellular Location Nucleus.
Citations (0)

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Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.


Greco-Stewart, V.S., et al. Virology 386(1):12-15(2009)
Yamamoto, K., et al. Mol. Cell. Biol. 24(14):6338-6349(2004)
Dynes, J.L., et al. J. Biochem. 135(3):429-438(2004)
Hirschler-Laszkiewicz, I., et al. J. Biol. Chem. 278(21):18953-18959(2003)
Yuan, X., et al. EMBO Rep. 3(11):1082-1087(2002)

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Cat# AP16145A
(40 western blots)
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