|Application ||WB, E|
|Other Accession||NP_647603.1, NP_005672.1|
|Calculated MW||52676 Da|
|Antigen Region||68-97 aa|
|Other Names||TATA box-binding protein-associated factor RNA polymerase I subunit A, RNA polymerase I-specific TBP-associated factor 48 kDa, TAFI48, TATA box-binding protein-associated factor 1A, TBP-associated factor 1A, Transcription factor SL1, Transcription initiation factor SL1/TIF-IB subunit A, TAF1A|
|Target/Specificity||This TAF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the N-terminal region of human TAF1A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TAF1A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre- initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.
Greco-Stewart, V.S., et al. Virology 386(1):12-15(2009)
Yamamoto, K., et al. Mol. Cell. Biol. 24(14):6338-6349(2004)
Dynes, J.L., et al. J. Biochem. 135(3):429-438(2004)
Hirschler-Laszkiewicz, I., et al. J. Biol. Chem. 278(21):18953-18959(2003)
Yuan, X., et al. EMBO Rep. 3(11):1082-1087(2002)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.