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KRT28 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - KRT28 Antibody (C-term) AP16179b
    All lanes : Anti-KRT28 Antibody (C-term) at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Hela whole cell lysate Lane 5: A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7Z3Y7
Other Accession NP_853513.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50567 Da
Antigen Region 432-460 aa
Additional Information
Gene ID 162605
Other Names Keratin, type I cytoskeletal 28, Cytokeratin-28, CK-28, Keratin-25D, K25D, Keratin-28, K28, Type I inner root sheath-specific keratin-K25irs4, KRT28 (HGNC:30842)
Target/Specificity This KRT28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 432-460 amino acids from the C-terminal region of human KRT28.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKRT28 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT28 (HGNC:30842)
Function Essential for the proper assembly of types I and II keratin protein complexes and the formation of keratin intermediate filaments in the inner root sheath (irs).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:A6BLY7}.
Tissue Location Strongly expressed in skin and scalp, and weak expression observed in thymus. In the hair follicle, expressed in Henle layer, Huxley layer and in the irs cuticle. Expression extends from the bulb region up to the point of differentiation into the three layers. Also present in the medulla of beard hair (at protein level).
Research Areas
Citations (0)

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This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21.


Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
Rogers, M.A., et al. Differentiation 72 (9-10), 527-540 (2004) :

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$ 295.00
$ 99.00
Cat# AP16179b
(40 western blots)
Availability: In Stock
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