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HIST1H2BB/HIST1H2BE Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HIST1H2BB/HIST1H2BE Antibody (N-term) AP16190A
    HIST1H2BB/HIST1H2BE Antibody (N-term) (Cat. #AP16190a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HIST1H2BB/HIST1H2BE antibody detected the HIST1H2BB/HIST1H2BE protein (arrow).
  • WB - HIST1H2BB/HIST1H2BE Antibody (N-term) AP16190A
    Western blot analysis of HIST1H2BB/HIST1H2BE (arrow) using rabbit polyclonal HIST1H2BB/HIST1H2BE Antibody (N-term) (Cat. #AP16190a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HIST1H2BB/HIST1H2BE gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P33778
Other Accession P57053, Q8CGP0, Q8N257, Q64524, Q16778, Q6DRA6, Q6DN03, Q00715, P62808, Q8CGP2, P23527, Q99877, Q32L48, P10854, Q99880, Q8CGP1, Q2PFX4, O60814, P06899, Q93079, P10853, P58876, Q6ZWY9, P62807, NP_066406.1
Reactivity Human
Predicted Mouse, Monkey, Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 13950 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 3018
Other Names Histone H2B type 1-B, Histone H2B1, Histone H2Bf, H2B/f, HIST1H2BB, H2BFF
Target/Specificity This HIST1H2BB/HIST1H2BE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2BB/HIST1H2BE.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST1H2BB/HIST1H2BE Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms H2BFF
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)

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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.


Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
Pavri, R., et al. Cell 125(4):703-717(2006)
Bonenfant, D., et al. Mol. Cell Proteomics 5(3):541-552(2006)
Zhu, B., et al. Mol. Cell 20(4):601-611(2005)
Golebiowski, F., et al. Mol. Cell. Biochem. 279 (1-2), 133-139 (2005) :

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Cat# AP16190A
(40 western blots)
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