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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   HMGCL Antibody (Center)   

HMGCL Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HMGCL Antibody (Center) AP16221c
    HMGCL Antibody (Center) (Cat. #AP16221c) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the HMGCL antibody detected the HMGCL protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35914
Other Accession Q8HXZ6, Q29448, NP_000182.2, NP_001159531.1
Reactivity Human
Predicted Bovine, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34360 Da
Antigen Region 161-190 aa
Additional Information
Gene ID 3155
Other Names Hydroxymethylglutaryl-CoA lyase, mitochondrial, HL, HMG-CoA lyase, 3-hydroxy-3-methylglutarate-CoA lyase, HMGCL
Target/Specificity This HMGCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from the Central region of human HMGCL.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHMGCL Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Key enzyme in ketogenesis (ketone body formation). Terminal step in leucine catabolism. Ketone bodies (beta- hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.
Cellular Location Mitochondrion matrix. Peroxisome. Note=Unprocessed form is peroxisomal
Tissue Location Highest expression in liver. Expressed in pancreas, kidey, intestine, testis, fibroblasts and lymphoblasts Very low expression in brain and skeletal muscle. The relative expression of isoform 2 (at mRNA level) is highest in heart (30%), skeletal muscle (22%), and brain (14%)
Research Areas
Citations (0)

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HMGCL belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].


Fu, Z., et al. J. Biol. Chem. 285(34):26341-26349(2010)
Pierron, S., et al. Arch Pediatr 17(1):10-13(2010)
Menao, S., et al. Hum. Mutat. 30 (3), E520-E529 (2009) :
Lin, W.D., et al. Clin. Chim. Acta 401 (1-2), 33-36 (2009) :
Carrasco, P., et al. Mol. Genet. Metab. 91(2):120-127(2007)

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$ 295.00
$ 99.00
Cat# AP16221c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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