|Application ||WB, E|
|Other Accession||O88339, Q80VP1, NP_037465.2, NP_001123544.1, NP_001123543.1|
|Calculated MW||60293 Da|
|Antigen Region||203-232 aa|
|Other Names||Epsin-1, EH domain-binding mitotic phosphoprotein, EPS-15-interacting protein 1, EPN1|
|Target/Specificity||This EPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-232 amino acids from the Central region of human EPN1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EPN1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.|
|Cellular Location||Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Membrane, clathrin-coated pit Note=Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity)|
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Provided below are standard protocols that you may find useful for product applications.
EPN1 is an endocytic accessory protein that interacts with EPS15 (MIM 600051), the alpha subunit of the clathrin adaptor AP2 (AP2A1; MIM 601026), and clathrin (see MIM 118960), as well as with other accessory proteins for the endocytosis of clathrin-coated vesicles.
Liu, Z., et al. J. Cell Biol. 186(4):473-480(2009) Kazazic, M., et al. Traffic 10(2):235-245(2009) Olsen, J.V., et al. Cell 127(3):635-648(2006) Olsen, J.V., et al. Cell 127(3):635-648(2006) Schmid, E.M., et al. PLoS Biol. 4 (9), E262 (2006) :
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