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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Mouse Cdk8 Antibody (Center)   

Mouse Cdk8 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse Cdk8 Antibody (Center) AP16262c
    Mouse Cdk8 Antibody (Center) (Cat. #AP16262c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Cdk8 antibody detected the Cdk8 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8R3L8
Other Accession P49336, Q8JH47, NP_705827.2
Reactivity Human
Predicted Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 53210 Da
Antigen Region 245-272 aa
Additional Information
Gene ID 264064
Other Names Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Cdk8
Target/Specificity This Mouse Cdk8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-272 amino acids from the Central region of mouse Cdk8.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Cdk8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Cdk8
Function Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)

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Background

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation (By similarity).

References

Alarcon, C., et al. Cell 139(4):757-769(2009)
Westerling, T., et al. Mol. Cell. Biol. 27(17):6177-6182(2007)
Jang, M.K., et al. Mol. Cell 19(4):523-534(2005)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)

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Cat# AP16262c
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(40 western blots)
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