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VCIP1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96JH7 |
| Other Accession | Q8CF97, Q8CDG3, NP_079330.2 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 134321 Da |
| Antigen Region | 124-151 aa |
| Gene ID | 80124 |
|---|---|
| Other Names | Deubiquitinating protein VCIP135, Valosin-containing protein p97/p47 complex-interacting protein 1, Valosin-containing protein p97/p47 complex-interacting protein p135, VCP/p47 complex-interacting 135-kDa protein, VCPIP1, KIAA1850, VCIP135 |
| Target/Specificity | This VCIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-151 amino acids from the N-terminal region of human VCIP1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | VCIP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | VCPIP1 {ECO:0000303|PubMed:32649882, ECO:0000312|HGNC:HGNC:30897} |
|---|---|
| Function | Deubiquitinating enzyme involved in DNA repair and reassembly of the Golgi apparatus and the endoplasmic reticulum following mitosis (PubMed:32649882). Necessary for VCP-mediated reassembly of Golgi stacks after mitosis (By similarity). Plays a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By similarity). Also involved in DNA repair following phosphorylation by ATM or ATR: acts by catalyzing deubiquitination of SPRTN, thereby promoting SPRTN recruitment to chromatin and subsequent proteolytic cleavage of covalent DNA-protein cross-links (DPCs) (PubMed:32649882). Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains (PubMed:23827681). |
| Cellular Location | Nucleus. Cytoplasm. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q8CF97}. Golgi apparatus, Golgi stack {ECO:0000250|UniProtKB:Q8CF97}. Note=Associated with Golgi stacks and endoplasmic reticulum (By similarity). Displays cytoplasmic to nuclear translocation in response to DNA-protein cross-links (DPCs)-inducing agents (PubMed:32649882). {ECO:0000250|UniProtKB:Q8CF97, ECO:0000269|PubMed:32649882} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
VCIP1 acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By similarity).
References
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Wang, Y., et al. J. Cell Biol. 164(7):973-978(2004)
Ficarro, S., et al. J. Biol. Chem. 278(13):11579-11589(2003)
Uchiyama, K., et al. J. Cell Biol. 159(5):855-866(2002)
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