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VCIP1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - VCIP1 Antibody (N-term) AP16265a
    VCIP1 Antibody (N-term) (Cat. #AP16265a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the VCIP1 antibody detected the VCIP1 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q96JH7
Other Accession Q8CF97, Q8CDG3, NP_079330.2
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 134321 Da
Antigen Region 124-151 aa
Additional Information
Gene ID 80124
Other Names Deubiquitinating protein VCIP135, Valosin-containing protein p97/p47 complex-interacting protein 1, Valosin-containing protein p97/p47 complex-interacting protein p135, VCP/p47 complex-interacting 135-kDa protein, VCPIP1, KIAA1850, VCIP135
Target/Specificity This VCIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-151 amino acids from the N-terminal region of human VCIP1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVCIP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VCPIP1
Synonyms KIAA1850, VCIP135
Function Acts as a deubiquitinating enzyme. Necessary for VCP- mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By similarity). Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains.
Cellular Location Endoplasmic reticulum. Golgi apparatus, Golgi stack. Note=Associated with Golgi stacks and endoplasmic reticulum.
Research Areas
Citations (0)

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Background

VCIP1 acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By similarity).

References

Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Wang, Y., et al. J. Cell Biol. 164(7):973-978(2004)
Ficarro, S., et al. J. Biol. Chem. 278(13):11579-11589(2003)
Uchiyama, K., et al. J. Cell Biol. 159(5):855-866(2002)

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$ 295.00
$ 99.00
Cat# AP16265a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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