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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SIGLEC8 Antibody (C-term)   

SIGLEC8 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SIGLEC8 Antibody (C-term) AP1626b
    The anti-Siglec8 C-term Pab (Cat. #AP1626b) is used in Western blot to detect Siglec8 in mouse liver tissue lysate (lnae 1) and in HL60 cell lysate (lane 2).
  • IHC-P - SIGLEC8 Antibody (C-term) AP1626b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NYZ4
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54042 Da
Antigen Region 373-406 aa
Additional Information
Gene ID 27181
Other Names Sialic acid-binding Ig-like lectin 8, Siglec-8, Sialoadhesin family member 2, SAF-2, SIGLEC8, SAF2
Target/Specificity This SIGLEC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-406 amino acids from the C-terminal region of human SIGLEC8.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIGLEC8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms SAF2
Function Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed specifically on eosinophils.
Research Areas
Citations (0)

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SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.


Foussias, G., et al., Biochem. Biophys. Res. Commun. 278(3):775-781 (2000).
Floyd, H., et al., J. Biol. Chem. 275(2):861-866 (2000).
Kikly, K.K., et al., J. Allergy Clin. Immunol. 105 (6 Pt 1), 1093-1100 (2000).

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$ 295.00
$ 99.00
Cat# AP1626b
(40 western blots)
Availability: In Stock
Bulk Size
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