SIGLEC9 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | Q9Y336 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 50082 Da |
Antigen Region | 385-415 aa |
Gene ID | 27180 |
---|---|
Other Names | Sialic acid-binding Ig-like lectin 9, Siglec-9, CDw329, Protein FOAP-9, CD329, SIGLEC9 |
Target/Specificity | This SIGLEC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-415 amino acids from the C-terminal region of human SIGLEC9. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SIGLEC9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SIGLEC9 |
---|---|
Function | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. |
Cellular Location | Membrane; Single-pass type I membrane protein. |
Tissue Location | Expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils). Found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon |
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Background
SIGLEC9 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This protein is expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils). It is found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon. SIGLEC9 contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
References
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). Zhang, J.Q., et al., J. Biol. Chem. 275(29):22121-22126 (2000). Foussias, G., et al., Genomics 67(2):171-178 (2000). Angata, T., et al., J. Biol. Chem. 275(29):22127-22135 (2000). Yousef, G.M., et al., Anticancer Res. 19 (4B), 2843-2852 (1999).
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