- CITATIONS: 1
|Application ||WB, E|
|Other Accession||NP_877577.1, NP_005907.3|
|Calculated MW||81308 Da|
|Antigen Region||290-318 aa|
|Other Names||DNA replication licensing factor MCM7, CDC47 homolog, P11-MCM3, MCM7, CDC47, MCM2|
|Target/Specificity||This MCM7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-318 amino acids from the N-terminal region of human MCM7.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MCM7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Lau, K.M., et al. Oncogene 29(40):5475-5489(2010)
Kim, D.W., et al. Mol. Biochem. Parasitol. 173(1):10-16(2010)
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Rojiani, M.V., et al. Appl. Immunohistochem. Mol. Morphol. 18(3):278-282(2010)
Poliseno, L., et al. Sci Signal 3 (117), RA29 (2010) :
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