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Erythropoietin Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Erythropoietin Antibody (N-term) AP16337a
    Anti-Erythropoietin Antibody (N-term) at 1:2000 dilution + Rat kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Erythropoietin Antibody (N-term) AP16337a
    Anti-Erythropoietin Antibody (N-term) at 1:2000 dilution + Recombinant human EPO protein Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Erythropoietin Antibody (N-term) AP16337a
    All lanes : Anti-Erythropoietin Antibody (N-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Erythropoietin Antibody (N-term) AP16337a
    All lanes : Anti-Erythropoietin Antibody (N-term) at 1:2000 dilution Lane 1: H. kidney whole cell lysate Lane 2: H. liver whole cell lysate Lane 3: M. liver whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P01588
Other Accession P07865, NP_000790.2
Reactivity Human, Mouse, Rat
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 21307 Da
Antigen Region 28-48 aa
Additional Information
Gene ID 2056
Other Names Erythropoietin, Epoetin, EPO
Target/Specificity This Erythropoietin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-48 amino acids of human Erythropoietin.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsErythropoietin Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPO
Function Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
Cellular Location Secreted.
Tissue Location Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals
Research Areas
Citations (0)

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Background

EPO is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq].

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Kristensen, P.L., et al. Eur. J. Endocrinol. 163(3):391-398(2010)
Kim, M.H., et al. Mol. Biol. (Mosk.) 44(4):656-663(2010)
Carraway, M.S., et al. Circ. Res. 106(11):1722-1730(2010)
Shi, Z., et al. Mol. Cancer Res. 8(4):615-626(2010)

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$ 295.00
$ 99.00
Cat# AP16337a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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