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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ISL1 Antibody (Center)   

ISL1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ISL1 Antibody (Center) AP16352c
    ISL1 Antibody (Center) (Cat. #AP16352c) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ISL1 antibody detected the ISL1 protein (arrow).
  • WB - ISL1 Antibody (Center) AP16352c
    ISL1 Antibody (Center) (Cat. #AP16352c) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the ISL1 antibody detected the ISL1 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P61371
Other Accession P61374, P61372, P53405, P50211, NP_002193.2
Reactivity Human, Mouse
Predicted Chicken, Zebrafish, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39036 Da
Antigen Region 156-185 aa
Additional Information
Gene ID 3670
Other Names Insulin gene enhancer protein ISL-1, Islet-1, ISL1
Target/Specificity This ISL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the Central region of human ISL1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsISL1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ISL1
Function DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P61372}.
Tissue Location Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain
Research Areas
Citations (0)

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Background

ISL1 is a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

References

De Luca, A., et al. Clin. Genet. (2010) In press :
Davis, O.S., et al. Behav. Genet. (2010) In press :
Genead, R., et al. Stem Cell Res 4(1):69-76(2010)
Stevens, K.N., et al. PLoS ONE 5 (5), E10855 (2010) :
Zhang, H., et al. J. Mol. Biol. 392(3):566-577(2009)

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Cat# AP16352c
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(40 western blots)
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