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SIAH1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SIAH1 Antibody (Center) AP16396c
    SIAH1 Antibody (Center) (Cat. #AP16396c) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SIAH1 antibody detected the SIAH1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8IUQ4
Other Accession NP_003022.3, NP_001006611.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31123 Da
Antigen Region 92-120 aa
Additional Information
Gene ID 6477
Other Names E3 ubiquitin-protein ligase SIAH1, 632-, Seven in absentia homolog 1, Siah-1, Siah-1a, SIAH1, HUMSIAH
Target/Specificity This SIAH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-120 amino acids from the Central region of human SIAH1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIAH1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIAH1
Synonyms HUMSIAH
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S- nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins.
Cellular Location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear
Tissue Location Widely expressed at a low level. Down- regulated in advanced hepatocellular carcinomas
Research Areas
Citations (0)

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SIAH1 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq].


Wu, H., et al. Biochem. Biophys. Res. Commun. 397(3):391-396(2010)
Wen, Y.Y., et al. Mol. Carcinog. 49(5):440-449(2010)
Dimitrova, Y.N., et al. J. Biol. Chem. 285(18):13507-13516(2010)
Bruzzoni-Giovanelli, H., et al. J. Exp. Clin. Cancer Res. 29, 10 (2010) :
Xie, W., et al. J. Cell. Mol. Med. 13 (8B), 1719-1727 (2009) :

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$ 295.00
$ 99.00
Cat# AP16396c
(40 western blots)
Availability: In Stock
Bulk Size
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