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ENOSF1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ENOSF1 Antibody (N-term) AP16426A
    ENOSF1 Antibody (N-term) (Cat. #AP16426a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ENOSF1 antibody detected the ENOSF1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q7L5Y1
Other Accession NP_001119595.1, NP_974487.1, NP_059982.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 49786 Da
Antigen Region 63-92 aa
Additional Information
Gene ID 55556
Other Names Mitochondrial enolase superfamily member 1, Antisense RNA to thymidylate synthase, rTS, L-fuconate dehydratase, ENOSF1, RTS, TYMSAS
Target/Specificity This ENOSF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human ENOSF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsENOSF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENOSF1
Synonyms RTS, TYMSAS
Function Plays a role in the catabolism of L-fucose, a sugar that is part of the carbohydrates that are attached to cellular glycoproteins. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion (PubMed:24697329).
Cellular Location Mitochondrion.
Research Areas
Citations (0)
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Background

ENOSF1 was originally identified as a naturally occurring antisense transcript to the human thymidylate synthase gene. Alternate splice variants have been described, one of which (named rTSalpha) represents an alternate 3'UTR that is complementary to the 3'UTR and terminal intron of the thymidylate synthase (TS) RNA and down-regulates TS expression. Other transcript variants (rTSbeta and rTSgamma) do not overlap the TS locus. The function of this gene appears to be primarily to regulate expression of the TS locus both via the antisense transcript as well as through the encoded proteins. [provided by RefSeq].

References

Giusti, B., et al. Thromb. Haemost. 104(2):231-242(2010)
Wang, Y., et al. J. Hum. Genet. 55(8):490-494(2010)
Cross, D.S., et al. BMC Genet. 11, 51 (2010) :
Giusti, B., et al. J. Med. Genet. 45(11):721-730(2008)
Giusti, B., et al. Biochem. Genet. 46 (7-8), 406-423 (2008) :

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$ 182.50
$ 70.00
Cat# AP16426A
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