|Application ||WB, E|
|Calculated MW||88829 Da|
|Antigen Region||59-88 aa|
|Other Names||Centromere/kinetochore protein zw10 homolog, ZW10|
|Target/Specificity||This ZW10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the N-terminal region of human ZW10.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZW10 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:11590237, PubMed:15485811, PubMed:15824131). Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER (PubMed:15029241).|
|Cellular Location||Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle Note=Dynamic pattern of localization during the cell cycle. In most cells at interphase, present diffusely in the cytoplasm (PubMed:15029241). In prometaphase, associated with the kinetochore. At metaphase, detected both at the kinetochores and, most prominently, at the spindle, particularly at the spindle poles. In very early anaphase, detected on segregating kinetochores. In late anaphase and telophase, accumulates at the spindle midzone (PubMed:11590237).|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase.
Chan, Y.W., et al. J. Cell Biol. 185(5):859-874(2009)
Aoki, T., et al. Mol. Biol. Cell 20(11):2639-2649(2009)
Inoue, M., et al. Genes Cells 13(8):905-914(2008)
Famulski, J.K., et al. J. Cell Biol. 180(3):507-520(2008)
Famulski, J.K., et al. Curr. Biol. 17(24):2143-2149(2007)
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