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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   VPS11 Antibody (C-term)   

VPS11 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - VPS11 Antibody (C-term) AP16504b
    VPS11 Antibody (C-term) (Cat. #AP16504b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the VPS11 antibody detected the VPS11 protein (arrow).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9H270
Other Accession Q91W86, NP_068375.3
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 107837 Da
Antigen Region 772-800 aa
Additional Information
Gene ID 55823
Other Names Vacuolar protein sorting-associated protein 11 homolog, hVPS11, RING finger protein 108, VPS11, RNF108
Target/Specificity This VPS11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 772-800 amino acids from the C-terminal region of human VPS11.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVPS11 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VPS11
Synonyms RNF108
Function Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25266290, PubMed:25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:25783203). Involved in cargo transport from early to late endosomes and required for the transition from early to late endosomes (PubMed:21148287). Involved in the retrograde Shiga toxin transport (PubMed:23593995).
Cellular Location Endosome. Late endosome membrane; Peripheral membrane protein; Cytoplasmic side Lysosome membrane; Peripheral membrane protein; Cytoplasmic side Early endosome {ECO:0000269|PubMed:21148287, ECO:0000305} Cytoplasmic vesicle. Cytoplasmic vesicle, autophagosome. Cytoplasmic vesicle, clathrin-coated vesicle
Tissue Location Ubiquitous. Expression was highest in heart and low in lung
Research Areas
Citations (0)

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Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

References

Bailey, S.D., et al. Diabetes Care (2010) In press :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Zhu, G.D., et al. Mol. Biol. Cell 20(4):1223-1240(2009)
Wan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 101(44):15724-15729(2004)
Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)

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$ 295.00
$ 99.00
Cat# AP16504b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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