|Application ||WB, E|
|Calculated MW||37099 Da|
|Antigen Region||287-315 aa|
|Other Names||Mas-related G-protein coupled receptor member X2, MRGPRX2, MRGX2|
|Target/Specificity||This MRGPRX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-315 amino acids from the C-terminal region of human MRGPRX2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MRGPRX2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core (PubMed:25517090). Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo- allergic reactions characterized by histamine release, inflammation and airway contraction (By similarity). Acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Mainly expressed in mast cells. Has a limited expression profile, both peripheral and within the central nervous system, with highest levels in dorsal root ganglion (PubMed:12915402). Detected in blood vessels, scattered lymphocytes, and gastrointestinal ganglia (at protein level) (PubMed:16161007).|
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Provided below are standard protocols that you may find useful for product applications.
MRGPRX2 is the orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin-stimulated cAMP levels, suggesting that this receptor is G(q)-coupled.
Gembardt, F., et al. Mol. Cell. Biochem. 319 (1-2), 115-123 (2008) :
Yang, S., et al. Gene 352, 30-35 (2005) :
Robas, N., et al. J. Biol. Chem. 278(45):44400-44404(2003)
Takeda, S., et al. FEBS Lett. 520 (1-3), 97-101 (2002) :
Dong, X., et al. Cell 106(5):619-632(2001)
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