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ISL2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ISL2 Antibody (N-term) AP16528b
    Fluorescent image of HUVEC cell stained with ISL2 Antibody (N-term)(Cat#AP16528b).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ISL2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).ISL2 immunoreactivity is localized to Nucleus significantly.
  • WB - ISL2 Antibody (N-term) AP16528b
    ISL2 Antibody (N-term) (Cat. #AP16528b) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ISL2 antibody detected the ISL2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession Q96A47
Other Accession P50480, Q9CXV0, NP_665804.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 1-30 aa
Additional Information
Other Names Insulin gene enhancer protein ISL-2, Islet-2, ISL2
Target/Specificity This ISL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ISL2.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsISL2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ISL2
Function Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108} EMBL; AK001022; BAG50849.1; -; mRNA EMBL; CH471136; EAW99220.1; -; Genomic_DNA EMBL; BC011967; AAH11967.1; -; mRNA EMBL; BC012136; AAH12136.1; -; mRNA CCDS; CCDS10290.1; - RefSeq; NP_665804.1; NM_145805.2 UniGene; Hs.444677; - ProteinModelPortal; Q96A47; - SMR; Q96A47; - BioGrid; 122318; 12 IntAct; Q96A47; 1 STRING; 9606.ENSP00000290759; - iPTMnet; Q96A47; - PhosphoSitePlus; Q96A47; - BioMuta; ISL2; - DMDM; 20978495; - EPD; Q96A47; - MaxQB; Q96A47; - PaxDb; Q96A47; - PeptideAtlas; Q96A47; - PRIDE; Q96A47; - ProteomicsDB; 75911; - DNASU; 64843; - Ensembl; ENST00000290759; ENSP00000290759; ENSG00000159556 GeneID; 64843; - KEGG; hsa:64843; - UCSC; uc002bbw.2; human CTD; 64843; - EuPathDB; HostDB:ENSG00000159556.9; - GeneCards; ISL2; - HGNC; HGNC:18524; ISL2 HPA; HPA075192; - MIM; 609481; gene neXtProt; NX_Q96A47; - OpenTargets; ENSG00000159556; - PharmGKB; PA38566; - eggNOG; KOG0490; Eukaryota eggNOG; ENOG410YIJ3; LUCA GeneTree; ENSGT00760000118921; - HOGENOM; HOG000236304; - HOVERGEN; HBG004671; - InParanoid; Q96A47; - KO; K18492; - OMA; RHDSAVQ; - OrthoDB; EOG091G09L9; - PhylomeDB; Q96A47; - TreeFam; TF315442; - SIGNOR; Q96A47; - GenomeRNAi; 64843; - PRO; PR:Q96A47; - Proteomes; UP000005640; Chromosome 15 Bgee; ENSG00000159556; - CleanEx; HS_ISL2; - ExpressionAtlas; Q96A47; baseline and differential Genevisible; Q96A47; HS GO; GO:0005634; C:nucleus; IC:BHF-UCL GO; GO:0003677; F:DNA binding; ISS:BHF-UCL GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW GO; GO:0000981; F:RNA polymerase II transcription factor activity, sequence-specific DNA binding; ISA:NTNU_SB GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro GO; GO:0045665; P:negative regulation of neuron differentiation; IEA:Ensembl GO; GO:0048666; P:neuron development; TAS:BHF-UCL GO; GO:0048935; P:peripheral nervous system neuron development; TAS:BHF-UCL GO; GO:0031290; P:retinal ganglion cell axon guidance; IEA:Ensembl GO; GO:0021520; P:spinal cord motor neuron cell fate specification; IEA:Ensembl GO; GO:0021524; P:visceral motor neuron differentiation; IEA:Ensembl CDD; cd00086; homeodomain; 1 InterPro; IPR009057; Homeobox-like_sf InterPro; IPR017970; Homeobox_CS InterPro; IPR001356; Homeobox_dom InterPro; IPR001781; Znf_LIM Pfam; PF00046; Homeobox; 1 Pfam; PF00412; LIM; 2 SMART; SM00389; HOX; 1 SMART; SM00132; LIM; 2 SUPFAM; SSF46689; SSF46689; 1 PROSITE; PS00027; HOMEOBOX_1; 1 PROSITE; PS50071; HOMEOBOX_2; 1 PROSITE; PS00478; LIM_DOMAIN_1; 2 PROSITE; PS50023; LIM_DOMAIN_2; 2 1: Evidence at protein level; Complete proteome; Developmental protein; DNA-binding; Homeobox; LIM domain; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Zinc CHAIN 1 359 Insulin gene enhancer protein ISL-2 /FTId=PRO_0000075752 DOMAIN 25 86 LIM zinc-binding 1. {ECO:0000255|PROSITE- ProRule:PRU00125} DOMAIN 87 149 LIM zinc-binding 2. {ECO:0000255|PROSITE- ProRule:PRU00125} DNA_BIND 191 250 Homeobox. {ECO:0000255|PROSITE- ProRule:PRU00108} REGION 272 301 LIM-binding domain (LID). MOD_RES 154 154 Phosphoserine MOD_RES 157 157 Phosphoserine MOD_RES 279 279 Phosphoserine SEQUENCE 359 AA; 39768 MW; 2162FB3D430EE4BE CRC64; MVDIIFHYPF LGAMGDHSKK KPGTAMCVGC GSQIHDQFIL RVSPDLEWHA ACLKCAECSQ YLDETCTCFV RDGKTYCKRD YVRLFGIKCA KCQVGFSSSD LVMRARDSVY HIECFRCSVC SRQLLPGDEF SLREHELLCR ADHGLLLERA AAGSPRSPGP LPGARGLHLP DAGSGRQPAL RPHVHKQTEK TTRVRTVLNE KQLHTLRTCY AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKKS ILMKQLQQQQ HSDKTSLQGL TGTPLVAGSP IRHENAVQGS AVEVQTYQPP WKALSEFALQ SDLDQPAFQQ LVSFSESGSL GNSSGSDVTS LSSQLPDTPN SMVPSPVET
Research Areas
Citations (0)

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Background

ISL2 is a transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways (By similarity).

References

Li, Y., et al. Mol. Biol. Rep. 34(1):19-26(2007)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Jurata, L.W., et al. J. Biol. Chem. 273(6):3152-3157(1998)

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Cat# AP16528b
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