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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PLEKHF1 Antibody (N-term)   

PLEKHF1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PLEKHF1 Antibody (N-term) AP16531a
    Anti-PLEKHF1 Antibody (N-term) at 1:1000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q96S99
Other Accession NP_077286.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31195 Da
Antigen Region 51-79 aa
Additional Information
Gene ID 79156
Other Names Pleckstrin homology domain-containing family F member 1, PH domain-containing family F member 1, Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains, Apoptosis-inducing protein, PH and FYVE domain-containing protein 1, Phafin-1, Zinc finger FYVE domain-containing protein 15, PLEKHF1, APPD, LAPF, ZFYVE15
Target/Specificity This PLEKHF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human PLEKHF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPLEKHF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLEKHF1
Synonyms APPD, LAPF, ZFYVE15
Function May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase- independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.
Cellular Location Nucleus. Cytoplasm, perinuclear region Lysosome. Note=Translocates to lysosome during apoptosis
Tissue Location Highly expressed in heart and skeletal muscle. Weakly expressed in brain, thymus, spleen, kidney, liver, small intestine, placenta and lung.
Research Areas
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Background

PLEKHF1 may induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.

References

Chen, W., et al. J. Biol. Chem. 280(49):40985-40995(2005)

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$ 295.00
$ 99.00
Cat# AP16531a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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