PLEKHF1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q96S99 |
Other Accession | NP_077286.3 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 31195 Da |
Antigen Region | 51-79 aa |
Gene ID | 79156 |
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Other Names | Pleckstrin homology domain-containing family F member 1, PH domain-containing family F member 1, Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains, Apoptosis-inducing protein, PH and FYVE domain-containing protein 1, Phafin-1, Zinc finger FYVE domain-containing protein 15, PLEKHF1, APPD, LAPF, ZFYVE15 |
Target/Specificity | This PLEKHF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human PLEKHF1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PLEKHF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PLEKHF1 |
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Synonyms | APPD, LAPF, ZFYVE15 |
Function | May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8. |
Cellular Location | Nucleus. Cytoplasm, perinuclear region. Lysosome. Note=Translocates to lysosome during apoptosis |
Tissue Location | Highly expressed in heart and skeletal muscle. Weakly expressed in brain, thymus, spleen, kidney, liver, small intestine, placenta and lung. |
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Provided below are standard protocols that you may find useful for product applications.
Background
PLEKHF1 may induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.
References
Chen, W., et al. J. Biol. Chem. 280(49):40985-40995(2005)
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