|Application ||WB, E|
|Calculated MW||28943 Da|
|Antigen Region||104-133 aa|
|Other Names||HLA class II histocompatibility antigen, DM beta chain, MHC class II antigen DMB, Really interesting new gene 7 protein, HLA-DMB, DMB, RING7|
|Target/Specificity||This HLA-DMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the Central region of human HLA-DMB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HLA-DMB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.|
|Cellular Location||Late endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=Localizes to late endocytic compartment. Associates with lysosome membranes|
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Provided below are standard protocols that you may find useful for product applications.
HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
Rinderknecht, C.H., et al. Immunology 131(1):18-32(2010)
Bailey, S.D., et al. Diabetes Care (2010) In press :
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Ferrante, A., et al. J. Immunol. 184(3):1153-1158(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
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