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DEF6 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9H4E7 |
| Other Accession | Q8C2K1, NP_071330.3 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 73910 Da |
| Antigen Region | 500-528 aa |
| Gene ID | 50619 |
|---|---|
| Other Names | Differentially expressed in FDCP 6 homolog, DEF-6, IRF4-binding protein, DEF6, IBP |
| Target/Specificity | This DEF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 500-528 amino acids from the C-terminal region of human DEF6. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DEF6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DEF6 |
|---|---|
| Synonyms | IBP |
| Function | Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42 (PubMed:12651066, PubMed:15023524). Can regulate cell morphology in cooperation with activated RAC1 (By similarity). Involved in immune homeostasis by ensuring proper trafficking and availability of T-cell regulator CTLA-4 at T-cell surface (PubMed:31308374). Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling (By similarity). |
| Cellular Location | Cytoplasm. Cell membrane Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell projection, filopodium. Note=Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate (PubMed:15023524). Binds to actin filaments (PubMed:15023524) |
| Tissue Location | Broadly expressed in the immune system. Highly expressed in T cells (PubMed:31308374). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).
References
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :
Zhang, Z., et al. Cancer Epidemiol 33(2):130-136(2009)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009)
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