|Application ||WB, E|
|Other Accession||Q8C2K1, NP_071330.3|
|Calculated MW||73910 Da|
|Antigen Region||500-528 aa|
|Other Names||Differentially expressed in FDCP 6 homolog, DEF-6, IRF4-binding protein, DEF6, IBP|
|Target/Specificity||This DEF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 500-528 amino acids from the C-terminal region of human DEF6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DEF6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling (By similarity).|
|Cellular Location||Cytoplasm. Cell membrane. Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell projection, filopodium. Note=Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate (PubMed:15023524). Binds to actin filaments (PubMed:15023524)|
|Tissue Location||Broadly expressed in the immune system and can be detected in T and B-cells.|
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Provided below are standard protocols that you may find useful for product applications.
DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :
Zhang, Z., et al. Cancer Epidemiol 33(2):130-136(2009)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009)
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