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ATF3 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | P18847 |
| Other Accession | P29596, Q60765, Q2KII1, NP_001025458.1, NP_001665.1 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 20576 Da |
| Antigen Region | 140-168 aa |
| Gene ID | 467 |
|---|---|
| Other Names | Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3, ATF3 |
| Target/Specificity | This ATF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the C-terminal region of human ATF3. |
| Dilution | WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ATF3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ATF3 {ECO:0000303|PubMed:7515060, ECO:0000312|HGNC:HGNC:785} |
|---|---|
| Function | This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:12034827} |
Provided below are standard protocols that you may find useful for product applications.
Background
Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Lee, S.H., et al. Oncogene 29(37):5182-5192(2010)
Park, H.J., et al. Biochem. Biophys. Res. Commun. 400(1):72-77(2010)
Wu, X., et al. Nature 465(7296):368-372(2010)
Koh, I.U., et al. FEBS J. 277(10):2304-2317(2010)
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