|Application ||WB, E|
|Other Accession||NP_001035982.1, NP_112194.2|
|Calculated MW||136926 Da|
|Antigen Region||1061-1090 aa|
|Other Names||Protein diaphanous homolog 3, Diaphanous-related formin-3, DRF3, MDia2, DIAPH3, DIAP3|
|Target/Specificity||This DIAPH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061-1090 amino acids from the C-terminal region of human DIAPH3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DIAPH3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).|
|Cellular Location||Cytoplasm, cytosol Note=During mitosis, co-localizes with the actin-rich cleavage furrow and with the microtubule-rich central spindle during cytokinesis|
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Provided below are standard protocols that you may find useful for product applications.
DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).
DeWard, A.D., et al. J. Biol. Chem. 284(30):20061-20069(2009)
Block, J., et al. J Microsc 231(3):506-517(2008)
Beli, P., et al. Nat. Cell Biol. 10(7):849-857(2008)
Yang, C., et al. PLoS Biol. 5 (11), E317 (2007) :
Gupton, S.L., et al. J. Cell. Sci. 120 (PT 19), 3475-3487 (2007) :
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