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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   DIAPH3 Antibody (C-term)   

DIAPH3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DIAPH3 Antibody (C-term) AP16587b
    DIAPH3 Antibody (C-term) (Cat. #AP16587b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DIAPH3 antibody detected the DIAPH3 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NSV4
Other Accession NP_001035982.1, NP_112194.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 136926 Da
Antigen Region 1061-1090 aa
Additional Information
Gene ID 81624
Other Names Protein diaphanous homolog 3, Diaphanous-related formin-3, DRF3, MDia2, DIAPH3, DIAP3
Target/Specificity This DIAPH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061-1090 amino acids from the C-terminal region of human DIAPH3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDIAPH3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DIAPH3
Synonyms DIAP3
Function Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).
Cellular Location Cytoplasm, cytosol Note=During mitosis, co-localizes with the actin-rich cleavage furrow and with the microtubule-rich central spindle during cytokinesis
Research Areas
Citations (0)

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Background

DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).

References

DeWard, A.D., et al. J. Biol. Chem. 284(30):20061-20069(2009)
Block, J., et al. J Microsc 231(3):506-517(2008)
Beli, P., et al. Nat. Cell Biol. 10(7):849-857(2008)
Yang, C., et al. PLoS Biol. 5 (11), E317 (2007) :
Gupton, S.L., et al. J. Cell. Sci. 120 (PT 19), 3475-3487 (2007) :

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$ 295.00
$ 99.00
Cat# AP16587b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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