SRP54 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | P61011 |
Other Accession | Q6AYB5, P14576, Q4R965, Q2T9U1, NP_003127.1, NP_001139754.1, Q7ZVN5 |
Reactivity | Human |
Predicted | Bovine, Zebrafish, Monkey, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 55705 Da |
Antigen Region | 346-374 aa |
Gene ID | 6729 |
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Other Names | Signal recognition particle 54 kDa protein, SRP54, SRP54 |
Target/Specificity | This SRP54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-374 amino acids from the C-terminal region of human SRP54. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SRP54 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SRP54 (HGNC:11301) |
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Function | Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER) (PubMed:34020957). As part of the SRP complex, associates with the SRP receptor (SR) component SRPRA to target secretory proteins to the endoplasmic reticulum membrane (PubMed:34020957). Binds to the signal sequence of presecretory proteins when they emerge from the ribosomes (PubMed:34020957). Displays basal GTPase activity, and stimulates reciprocal GTPase activation of the SR subunit SRPRA (PubMed:28972538, PubMed:34020957). Forms a guanosine 5'-triphosphate (GTP)-dependent complex with the SR subunit SRPRA (PubMed:34020957). SR compaction and GTPase mediated rearrangement of SR drive SRP-mediated cotranslational protein translocation into the ER (PubMed:34020957). Requires the presence of SRP9/SRP14 and/or SRP19 to stably interact with RNA (By similarity). Plays a role in proliferation and differentiation of granulocytic cells, neutrophils migration capacity and exocrine pancreas development (PubMed:28972538, PubMed:29914977). |
Cellular Location | Nucleus speckle. Cytoplasm Endoplasmic reticulum |
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Provided below are standard protocols that you may find useful for product applications.
Background
SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Yoshida, M., et al. Mol. Pharmacol. 73(3):987-994(2008)
Sakashita, E., et al. Mol. Cell. Biol. 24(3):1174-1187(2004)
Kuglstatter, A., et al. Nat. Struct. Biol. 9(10):740-744(2002)
Yin, J., et al. RNA 7(10):1389-1396(2001)
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