|Application ||WB, E|
|Other Accession||Q5XI50, NP_073737.1|
|Calculated MW||78051 Da|
|Antigen Region||67-95 aa|
|Other Names||E3 ubiquitin-protein ligase MARCH7, 632-, Axotrophin, Membrane-associated RING finger protein 7, Membrane-associated RING-CH protein VII, MARCH-VII, RING finger protein 177, MARCH7, AXOT, RNF177|
|Target/Specificity||This MARCH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-95 amino acids from the N-terminal region of human MARCH7.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MARCH7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.|
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Provided below are standard protocols that you may find useful for product applications.
MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 22.214.171.124). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]).
Szigyarto, C.A., et al. J. Histochem. Cytochem. 58(4):301-308(2010)
Nathan, J.A., et al. Traffic 9(7):1130-1145(2008)
Muthukumarana, P.A., et al. Int. Immunopharmacol. 6 (13-14), 1993-2001 (2006) :
Flierman, D., et al. Proc. Natl. Acad. Sci. U.S.A. 103(31):11589-11594(2006)
Lim, J., et al. Cell 125(4):801-814(2006)
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