|Application ||WB, E|
|Other Accession||Q9D3E6, NP_005853.2|
|Calculated MW||144427 Da|
|Antigen Region||540-568 aa|
|Other Names||Cohesin subunit SA-1, SCC3 homolog 1, Stromal antigen 1, STAG1, SA1|
|Target/Specificity||This STAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-568 amino acids from the Central region of human STAG1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||STAG1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.|
|Cellular Location||Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK1, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation|
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Provided below are standard protocols that you may find useful for product applications.
This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.
Chasman, D.I., et al. PLoS Genet. 5 (11), E1000730 (2009) :
Canudas, S., et al. J. Cell Biol. 187(2):165-173(2009)
Rubio, E.D., et al. Proc. Natl. Acad. Sci. U.S.A. 105(24):8309-8314(2008)
Watrin, E., et al. Curr. Biol. 16(9):863-874(2006)
Losada, A., et al. J. Cell. Sci. 118 (PT 10), 2133-2141 (2005) :
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