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ARL2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ARL2 Antibody (Center) AP16648c
    All lanes : Anti-ARL2 Antibody (Center) at 1:2000 dilution Lane 1: T47D whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ARL2 Antibody (Center) AP16648c
    Western blot analysis of ARL2 (arrow) using rabbit polyclonal ARL2 Antibody (Center) (Cat. #AP16648c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARL2 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P36404
Other Accession O08697, Q9D0J4, Q06849, Q2TA37, NP_001658.2
Reactivity Human
Predicted Bovine, Drosophila, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20878 Da
Antigen Region 54-82 aa
Additional Information
Gene ID 402
Other Names ADP-ribosylation factor-like protein 2, ARL2
Target/Specificity This ARL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-82 amino acids from the Central region of human ARL2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARL2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARL2
Function Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle.
Cellular Location Mitochondrion intermembrane space. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cytoplasm. Note=The complex formed with ARL2BP, ARL2 and SLC25A6 is expressed in mitochondria. The complex formed with ARL2BP, ARL2 and SLC25A4 is expressed in mitochondria (By similarity). Not detected in the Golgi, nucleus and on the mitotic spindle. Centrosome-associated throughout the cell cycle Not detected to interphase microtubules.
Research Areas
Citations (0)

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This gene encodes a small GTP-binding protein of the RAS superfamily which functions as an ADP-ribosylation factor (ARF). The encoded protein is one of a functionally distinct group of ARF-like genes.


Zhang, T., et al. Structure 17(4):602-610(2009)
Beghin, A., et al. Cell Cycle 7(19):3074-3082(2008)
Veltel, S., et al. FEBS Lett. 582(17):2501-2507(2008)
Shultz, T., et al. FASEB J. 22(1):168-182(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

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$ 295.00
$ 99.00
Cat# AP16648c
(40 western blots)
Availability: In Stock
Bulk Size
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