|Application ||WB, E|
|Calculated MW||19618 Da|
|Antigen Region||62-88 aa|
|Other Names||Histone H2B type W-T, H2B histone family member W testis-specific, H2BFWT|
|Target/Specificity||This H2BFWT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-88 amino acids from the Central region of human H2BFWT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||H2BFWT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Atypical histone H2B. Nucleosomes containing it are structurally and dynamically indistinguishable from those containing conventional H2B. However, unlike conventional H2B, does not recruit chromosome condensation factors and does not participate in the assembly of mitotic chromosomes. May be important for telomere function.|
|Cellular Location||Nucleus membrane. Chromosome|
|Tissue Location||Testis-specific. Present in sperm cells (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the H2B histone family that is specifically expressed in sperm nuclei. A polymorphism in the 5' UTR of this gene is associated with male infertility.
Lee, J., et al. J. Cell. Mol. Med. 13 (8B), 1942-1951 (2009) :
Boulard, M., et al. Mol. Cell. Biol. 26(4):1518-1526(2006)
Churikov, D., et al. Genomics 84(4):745-756(2004)
Churikov, D., et al. Cytogenet. Genome Res. 105 (2-4), 203-214 (2004) :
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