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H2BFWT Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - H2BFWT Antibody (Center) AP16663c
    Anti-H2BFWT Antibody (Center) at 1:1000 dilution + human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7Z2G1
Other Accession NP_001002916.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 19618 Da
Antigen Region 62-88 aa
Additional Information
Gene ID 158983
Other Names Histone H2B type W-T, H2B histone family member W testis-specific, H2BFWT
Target/Specificity This H2BFWT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-88 amino acids from the Central region of human H2BFWT.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsH2BFWT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Atypical histone H2B. Nucleosomes containing it are structurally and dynamically indistinguishable from those containing conventional H2B. However, unlike conventional H2B, does not recruit chromosome condensation factors and does not participate in the assembly of mitotic chromosomes. May be important for telomere function.
Cellular Location Nucleus membrane. Chromosome
Tissue Location Testis-specific. Present in sperm cells (at protein level).
Citations (0)

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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the H2B histone family that is specifically expressed in sperm nuclei. A polymorphism in the 5' UTR of this gene is associated with male infertility.


Lee, J., et al. J. Cell. Mol. Med. 13 (8B), 1942-1951 (2009) :
Boulard, M., et al. Mol. Cell. Biol. 26(4):1518-1526(2006)
Churikov, D., et al. Genomics 84(4):745-756(2004)
Churikov, D., et al. Cytogenet. Genome Res. 105 (2-4), 203-214 (2004) :

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$ 295.00
$ 99.00
Cat# AP16663c
(40 western blots)
Availability: In Stock
Bulk Size
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