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LEP Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - LEP Antibody (N-term) AP16666a
    LEP Antibody (N-term) (Cat. #AP16666a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the LEP antibody detected the LEP protein (arrow).
  • WB - LEP Antibody (N-term) AP16666a
    Western blot analysis of LEP (arrow) using rabbit polyclonal LEP Antibody (N-term) (Cat. #AP16666a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LEP gene.
  • IF - LEP Antibody (N-term) AP16666a
    Confocal immunofluorescent analysis of LEP Antibody (N-term)(Cat#AP16666a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • IHC-P - LEP Antibody (N-term) AP16666a
    LEP Antibody (N-term) (Cat. #AP16666a)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LEP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - LEP Antibody (N-term) AP16666a
    LEP Antibody (N-term) (Cat. #AP16666a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P41159
Other Accession P41160, NP_000221.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18641 Da
Antigen Region 8-37 aa
Additional Information
Gene ID 3952
Other Names Leptin, Obese protein, Obesity factor, LEP, OB, OBS
Target/Specificity This LEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-37 amino acids from the N-terminal region of human LEP.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLEP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LEP
Synonyms OB, OBS
Function May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Cellular Location Secreted.
Research Areas
Citations (0)

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This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq].


Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Qin, J., et al. J. Hum. Genet. 55(10):704-706(2010)
Friedlander, Y., et al. Ann. Hum. Genet. 74(5):387-398(2010)
Sarzynski, M.A., et al. Int J Obes (Lond) (2010) In press :
Iciek, R., et al. Ginekol. Pol. 81(8):571-577(2010)

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Cat# AP16666a
(40 western blots)
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