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TGFBI Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TGFBI Antibody (N-term) AP16708a
    Western blot analysis of TGFBI (arrow) using rabbit polyclonal TGFBI Antibody (N-term) (Cat. #AP16708a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGFBI gene.
  • FC - TGFBI Antibody (N-term) AP16708a
    TGFBI Antibody (N-term) (Cat. #AP16708a) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q15582
Other Accession Q95215, O11780, P82198, NP_000349.1, P55906
Reactivity Human
Predicted Bovine, Mouse, Pig, Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 74681 Da
Antigen Region 106-135 aa
Additional Information
Gene ID 7045
Other Names Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein, RGD-CAP, TGFBI, BIGH3
Target/Specificity This TGFBI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human TGFBI.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTGFBI Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TGFBI
Synonyms BIGH3
Function Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions (By similarity).
Cellular Location Secreted Secreted, extracellular space, extracellular matrix. Note=May be associated both with microfibrils and with the cell surface (PubMed:8077289)
Tissue Location Highly expressed in the corneal epithelium (PubMed:8077289). Expressed in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas (PubMed:8077289)
Research Areas
Citations (0)

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Background

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy.

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Edelstein, S.L., et al. Cornea 29(6):698-700(2010)
Romero, P., et al. Mol. Vis. 16, 1601-1609 (2010) :
Paliwal, P., et al. Mol. Vis. 16, 1429-1438 (2010) :
Yang, J., et al. Mol. Vis. 16, 1186-1193 (2010) :

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$ 295.00
$ 99.00
Cat# AP16708a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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