PARG Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q86W56 |
Other Accession | NP_003622.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 111110 Da |
Antigen Region | 821-849 aa |
Gene ID | 670;8505 |
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Other Names | Poly(ADP-ribose) glycohydrolase, PARG |
Target/Specificity | This PARG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 821-849 amino acids from the C-terminal region of human PARG. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PARG Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PARG {ECO:0000303|PubMed:14527731, ECO:0000312|HGNC:HGNC:8605} |
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Function | Poly(ADP-ribose) glycohydrolase that degrades poly(ADP- ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP- ribose) (PubMed:15450800, PubMed:21892188, PubMed:23102699, PubMed:23474714, PubMed:33186521, PubMed:34321462, PubMed:34019811). PARG acts both as an endo- and exoglycosidase, releasing poly(ADP- ribose) of different length as well as ADP-ribose monomers (PubMed:23102699, PubMed:23481255). It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated (PubMed:21892188, PubMed:23474714, PubMed:33186521). Poly(ADP-ribose) is synthesized after DNA damage is only present transiently and is rapidly degraded by PARG (PubMed:23102699, PubMed:34019811). Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress (PubMed:24906880). Responsible for the prevalence of mono-ADP-ribosylated proteins in cells, thanks to its ability to degrade poly(ADP-ribose) without cleaving the terminal protein-ribose bond (PubMed:33186521). Required for retinoid acid- dependent gene transactivation, probably by removing poly(ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR- dependent gene promoters (PubMed:23102699). Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). |
Cellular Location | [Isoform 1]: Nucleus Note=Colocalizes with PCNA at replication foci (PubMed:21398629) Relocalizes to the cytoplasm in response to DNA damage (PubMed:16460818). [Isoform 3]: Cytoplasm [Isoform 5]: Mitochondrion matrix |
Tissue Location | Ubiquitously expressed. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products.
References
Whatcott, C.J., et al. Exp. Cell Res. 315(20):3477-3485(2009)
Frizzell, K.M., et al. J. Biol. Chem. 284(49):33926-33938(2009)
Erdelyi, K., et al. FASEB J. 23(10):3553-3563(2009)
Ame, J.C., et al. J. Cell. Sci. 122 (PT 12), 1990-2002 (2009) :
Uchiumi, F., et al. Genes Cells 13(12):1229-1247(2008)
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