|Application ||WB, E|
|Other Accession||O35491, NP_003984.2|
|Calculated MW||60090 Da|
|Antigen Region||291-319 aa|
|Other Names||Dual specificity protein kinase CLK2, CDC-like kinase 2, CLK2|
|Target/Specificity||This CLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-319 amino acids from the Central region of human CLK2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CLK2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.|
|Cellular Location||Isoform 1: Nucleus. Nucleus speckle. Note=Inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle.|
|Tissue Location||Endothelial cells.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the CLK family of dual specificity protein kinases. CLK family members have been shown to interact with, and phosphorylate, serine- and arginine-rich (SR) proteins of the spliceosomal complex, which is a part of the regulatory mechanism that enables the SR proteins to control RNA splicing. Note that this gene is distinct from TELO2 gene (GeneID:9894), which shares CLK2 and hCLK2 symbol aliases in common with this gene, but encodes a protein that is involved in telomere length regulation.
Nam, S.Y., et al. J. Biol. Chem. 285(41):31157-31163(2010)
Eisenreich, A., et al. Circ. Res. 104(5):589-599(2009)
Rendtlew Danielsen, J.M., et al. J. Biol. Chem. 284(7):4140-4147(2009)
Castle, J.C., et al. Nat. Genet. 40(12):1416-1425(2008)
Jin, J., et al. Curr. Biol. 14(16):1436-1450(2004)
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