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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   SEPT2 Antibody (C-term)   

SEPT2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SEPT2 Antibody (C-term) AP16761b
    SEPT2 Antibody (C-term) (Cat. #AP16761b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SEPT2 antibody detected the SEPT2 protein (arrow).
  • WB - SEPT2 Antibody (C-term) AP16761b
    Western blot analysis of SEPT2 (arrow) using rabbit polyclonal SEPT2 Antibody (C-term) (Cat. #AP16761b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SEPT2 gene.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q15019
Other Accession Q91Y81, P42208, Q2NKY7, NP_001008492.1, NP_001008491.1
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 41487 Da
Antigen Region 280-309 aa
Additional Information
Gene ID 4735
Other Names Septin-2, Neural precursor cell expressed developmentally down-regulated protein 5, NEDD-5, SEPT2, DIFF6, KIAA0158, NEDD5
Target/Specificity This SEPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-309 amino acids from the C-terminal region of human SEPT2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSEPT2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SEPT2
Synonyms DIFF6, KIAA0158, NEDD5
Function Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cleavage furrow. Midbody. Cytoplasm, cell cortex. Cell projection, cilium membrane. Note=In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria. In epithelial cells, colocalizes with polyglutamylated tubulin around the trans-Golgi network, as well as juxatnuclear and proximal Golgi apparatus. Localizes at the base of the cilia near the morphological distinction between the cilia and plasma membranes
Tissue Location Widely expressed. Up-regulated in liver cancer.
Research Areas
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Background

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

References

Martins-de-Souza, D., et al. J Psychiatr Res (2010) In press :
Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009)
Shen, M., et al. Environ. Mol. Mutagen. 50(4):285-290(2009)
Mostowy, S., et al. J. Biol. Chem. 284(17):11613-11621(2009)

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$ 295.00
$ 99.00
Cat# AP16761b
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(40 western blots)
Availability: In Stock
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