|Application ||WB, E|
|Other Accession||NP_036309.1, NP_001135994.1|
|Calculated MW||50146 Da|
|Antigen Region||192-221 aa|
|Other Names||F-box only protein 5, Early mitotic inhibitor 1, FBXO5, EMI1, FBX5|
|Target/Specificity||This FBXO5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-221 amino acids from the Central region of human FBXO5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FBXO5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding.|
|Cellular Location||Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Note=In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified.
Lei, S.F., et al. J. Bone Miner. Res. (2010) In press :
Ma, H.T., et al. Mol. Cell. Biol. 29(24):6500-6514(2009)
Lee, J., et al. Mol. Biol. Cell 20(7):1891-1902(2009)
Gutgemann, I., et al. Mod. Pathol. 21(4):445-454(2008)
Iwai, H., et al. Cell 130(4):611-623(2007)
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