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>   home   >   Products   >   Primary Antibodies   >   Microbiology   >   RFX1 Antibody (C-term)   

RFX1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • IF - RFX1 Antibody (C-term) AP16766B
    Fluorescent image of U251 cell stained with RFX1 Antibody (C-term)(Cat#AP16766b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RFX1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).RFX1 immunoreactivity is localized to Nucleus significantly.
  • WB - RFX1 Antibody (C-term) AP16766B
    RFX1 Antibody (C-term) (Cat. #AP16766b) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RFX1 antibody detected the RFX1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P22670
Other Accession P48377, NP_002909.4
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 104758 Da
Antigen Region 896-925 aa
Additional Information
Gene ID 5989
Other Names MHC class II regulatory factor RFX1, Enhancer factor C, EF-C, Regulatory factor X 1, RFX, Transcription factor RFX1, RFX1
Target/Specificity This RFX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-925 amino acids from the C-terminal region of human RFX1.
Dilution IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRFX1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RFX1
Function Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter.
Cellular Location Nucleus.
Research Areas
Citations (0)

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This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes.


Zhao, M., et al. J. Autoimmun. 35(1):58-69(2010)
Purvis, T.L., et al. Gene 460 (1-2), 20-29 (2010) :
Hsu, Y.C., et al. J. Biol. Chem. 285(18):13885-13895(2010)
Seguin-Estevez, Q., et al. J. Immunol. 183(4):2545-2553(2009)
Zhang, Y., et al. Mol. Biol. (Mosk.) 43(1):77-84(2009)

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$ 265.00
$ 95.00
Cat# AP16766B
(40 western blots)
Availability: In Stock
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