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RFX1 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP16766b 400 µl (40 western blots) In Stock $ 265.00 Add to cart
AP16766b-ev 80 µl (8 western blots) In Stock $ 95.00 Add to cart
  • Specification
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RFX1 Antibody (C-term) - Product info

ApplicationIF, WB
  • Applications Legend:
  • WB=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • FC=Flow Cytometry
  • DB=Dot Blot
Primary AccessionP22670
Other AccessionP48377, NP_002909.4
ReactivityHuman
PredictedMouse
IsotypeRabbit Ig
Calculated MW104758 Da

RFX1 Antibody (C-term) - Additional info

Gene ID 5989
Other Names
RFX1; MHC class II regulatory factor RFX1; Enhancer factor C; Regulatory factor X 1; Transcription factor RFX1
Target/Specificity
This RFX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-925 amino acids from the C-terminal region of human RFX1.
Dilution
IF~~1:10~50
WB~~1:1000
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
RFX1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

RFX1 Antibody (C-term) - Protein Information

Name RFX1
Function
Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter.
Cellular Location
Nucleus.

RFX1 Antibody (C-term) - Related products

BP16766b: RFX1 Antibody (C-term) Blocking Peptide

RFX1 Antibody (C-term) - Application data

  • Fluorescent image of U251 cell stained with RFX1 Antibody (C-term)(Cat#AP16766b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RFX1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).RFX1 immunoreactivity is localized to Nucleus significantly.

  • RFX1 Antibody (C-term) (Cat. #AP16766b) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RFX1 antibody detected the RFX1 protein (arrow).

RFX1 Antibody (C-term) - Research Areas

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BACKGROUND

This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes.

REFERENCES

Zhao, M., et al. J. Autoimmun. 35(1):58-69(2010)
Purvis, T.L., et al. Gene 460 (1-2), 20-29 (2010) :
Hsu, Y.C., et al. J. Biol. Chem. 285(18):13885-13895(2010)
Seguin-Estevez, Q., et al. J. Immunol. 183(4):2545-2553(2009)
Zhang, Y., et al. Mol. Biol. (Mosk.) 43(1):77-84(2009)