RFX1 Antibody (C-term)
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
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(40 western blots)
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(8 western blots)
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RFX1 Antibody (C-term) - Product Information
|Application ||IF, WB|
|Other Accession||P48377, NP_002909.4|
|Calculated MW||104758 Da|
|Antigen Region||896-925 aa|
RFX1 Antibody (C-term) - Additional Information
|Gene ID 5989|
RFX1; MHC class II regulatory factor RFX1; Enhancer factor C; Regulatory factor X 1; Transcription factor RFX1
This RFX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-925 amino acids from the C-terminal region of human RFX1.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
RFX1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
RFX1 Antibody (C-term) - Protein Information
Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter.
RFX1 Antibody (C-term) - Related Products
RFX1 Antibody (C-term) - Research Areas
Fluorescent image of U251 cell stained with RFX1 Antibody (C-term)(Cat#AP16766b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RFX1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).RFX1 immunoreactivity is localized to Nucleus significantly.
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Provided below are standard protocols that you may find useful for product applications.
This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes.
Zhao, M., et al. J. Autoimmun. 35(1):58-69(2010)
Purvis, T.L., et al. Gene 460 (1-2), 20-29 (2010) :
Hsu, Y.C., et al. J. Biol. Chem. 285(18):13885-13895(2010)
Seguin-Estevez, Q., et al. J. Immunol. 183(4):2545-2553(2009)
Zhang, Y., et al. Mol. Biol. (Mosk.) 43(1):77-84(2009)