|Application ||WB, E|
|Other Accession||Q9Z321, NP_003926.1|
|Calculated MW||96662 Da|
|Antigen Region||826-854 aa|
|Other Names||DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1, TOP3B, TOP3B1|
|Target/Specificity||This TOP3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 826-854 amino acids from the C-terminal region of human TOP3B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TOP3B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.|
|Tissue Location||Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however, not all variants have been fully described.
Oliveira-Costa, J.P., et al. Hum. Pathol. 41(11):1624-1630(2010)
Lesch, K.P., et al. J Neural Transm 115(11):1573-1585(2008)
Cho, Y.H., et al. Biochim. Biophys. Acta 1679(3):272-278(2004)
Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :
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