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TOP3B Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TOP3B Antibody (C-term) AP16775b
    TOP3B Antibody (C-term) (Cat. #AP16775b) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TOP3B antibody detected the TOP3B protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95985
Other Accession Q9Z321, NP_003926.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 96662 Da
Antigen Region 826-854 aa
Additional Information
Gene ID 8940
Other Names DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1, TOP3B, TOP3B1
Target/Specificity This TOP3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 826-854 amino acids from the C-terminal region of human TOP3B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTOP3B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TOP3B
Synonyms TOP3B1
Function Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.
Tissue Location Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas
Citations (0)

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This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however, not all variants have been fully described.


Oliveira-Costa, J.P., et al. Hum. Pathol. 41(11):1624-1630(2010)
Lesch, K.P., et al. J Neural Transm 115(11):1573-1585(2008)
Cho, Y.H., et al. Biochim. Biophys. Acta 1679(3):272-278(2004)
Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :

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$ 295.00
$ 99.00
Cat# AP16775b
(40 western blots)
Availability: In Stock
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