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MYH9 Antibody (N-term Y158)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MYH9 Antibody (N-term Y158) AP16804a
    All lanes : Anti-MYH9 Antibody (N-term Y158) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 227 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MYH9 Antibody (N-term Y158) AP16804a
    MYH9 Antibody (N-term Y158) (Cat. #AP16804a) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the MYH9 antibody detected the MYH9 protein (arrow).
  • FC - MYH9 Antibody (N-term Y158) AP16804a
    MYH9 Antibody (N-term Y158) (Cat. #AP16804a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35579
Other Accession Q62812, Q8VDD5, P14105, NP_002464.1
Reactivity Human, Mouse
Predicted Chicken, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 226532 Da
Antigen Region 134-165 aa
Additional Information
Gene ID 4627
Other Names Myosin-9, Cellular myosin heavy chain, type A, Myosin heavy chain 9, Myosin heavy chain, non-muscle IIa, Non-muscle myosin heavy chain A, NMMHC-A, Non-muscle myosin heavy chain IIa, NMMHC II-a, NMMHC-IIA, MYH9
Target/Specificity This MYH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-165 amino acids from the N-terminal region of human MYH9.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMYH9 Antibody (N-term Y158) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYH9
Function Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10.
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Colocalizes with actin filaments at lamellipodia margins and at the leading edge of migrating cells.
Tissue Location In the kidney, expressed in the glomeruli. Also expressed in leukocytes.
Research Areas
Citations (0)

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This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.


Arii, J., et al. Nature 467(7317):859-862(2010)
Genovese, G., et al. Kidney Int. 78(7):698-704(2010)
Tzur, S., et al. Hum. Genet. 128(3):345-350(2010)
Bostrom, M.A., et al. Hum. Genet. 128(2):195-204(2010)
Oleksyk, T.K., et al. PLoS ONE 5 (7), E11474 (2010) :

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$ 295.00
$ 99.00
Cat# AP16804a
(40 western blots)
Availability: In Stock
Bulk Size
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