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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   NPRL2 Antibody (C-term)   

NPRL2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NPRL2 Antibody (C-term) AP16818B
    NPRL2 Antibody (C-term) (Cat. #AP16818b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NPRL2 antibody detected the NPRL2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8WTW4
Other Accession Q9WUE4, NP_006536.3
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43658 Da
Antigen Region 346-373 aa
Additional Information
Gene ID 10641
Other Names Nitrogen permease regulator 2-like protein, NPR2-like protein, Gene 21 protein, G21 protein, Tumor suppressor candidate 4, NPRL2, TUSC4
Target/Specificity This NPRL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-373 amino acids from the C-terminal region of human NPRL2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNPRL2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NPRL2
Synonyms TUSC4
Function Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at 'Tyr-9', 'Tyr-373' and 'Tyr-376' residues. May act as a tumor suppressor. Suppresses cell growth and enhances sensitivity to various anticancer drugs. As a component of the GATOR1 complex, inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling.
Tissue Location Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in most lung cancer cell lines tested
Research Areas
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Background

Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at the Tyr-9 Tyr-373 and Tyr-376 residues. May act as a tumor suppressor. Suppresses cell growth and enhanced sensitivity to various anticancer drugs.

References

Spielewoy, N., et al. Eukaryotic Cell 9(4):592-601(2010)
Otani, S., et al. J Surg Oncol 100(5):358-363(2009)
Neklesa, T.K., et al. PLoS Genet. 5 (6), E1000515 (2009) :
Anedchenko, E.A., et al. Mol. Biol. (Mosk.) 42(6):965-976(2008)
Kurata, A., et al. Cancer Sci. 99(9):1827-1834(2008)

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Cat# AP16818B
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Quantity:
(40 western blots)
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