|Application ||WB, E|
|Other Accession||Q9WUE4, NP_006536.3|
|Calculated MW||43658 Da|
|Antigen Region||346-373 aa|
|Other Names||Nitrogen permease regulator 2-like protein, NPR2-like protein, Gene 21 protein, G21 protein, Tumor suppressor candidate 4, NPRL2, TUSC4|
|Target/Specificity||This NPRL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-373 amino acids from the C-terminal region of human NPRL2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NPRL2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at 'Tyr-9', 'Tyr-373' and 'Tyr-376' residues. May act as a tumor suppressor. Suppresses cell growth and enhances sensitivity to various anticancer drugs. As a component of the GATOR1 complex, inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling.|
|Tissue Location||Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in most lung cancer cell lines tested|
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Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at the Tyr-9 Tyr-373 and Tyr-376 residues. May act as a tumor suppressor. Suppresses cell growth and enhanced sensitivity to various anticancer drugs.
Spielewoy, N., et al. Eukaryotic Cell 9(4):592-601(2010)
Otani, S., et al. J Surg Oncol 100(5):358-363(2009)
Neklesa, T.K., et al. PLoS Genet. 5 (6), E1000515 (2009) :
Anedchenko, E.A., et al. Mol. Biol. (Mosk.) 42(6):965-976(2008)
Kurata, A., et al. Cancer Sci. 99(9):1827-1834(2008)
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