|Application ||WB, E|
|Calculated MW||70973 Da|
|Antigen Region||277-306 aa|
|Other Names||E3 ubiquitin/ISG15 ligase TRIM25, 632n3, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147, TRIM25, EFP, RNF147, ZNF147|
|Target/Specificity||This TRIM25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the Central region of human TRIM25.|
|Precautions||TRIM25 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||EFP, RNF147, ZNF147|
|Function||Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).|
|Cellular Location||Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies|
|Tissue Location||Expressed in breast tumors (at protein level). Ubiquitous.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Dai, H., et al. Oncol. Rep. 23(3):795-799(2010)
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :
Gack, M.U., et al. Cell Host Microbe 5(5):439-449(2009)
Ludwig, S., et al. Cell Host Microbe 5(5):420-421(2009)
Dai, H., et al. Oncol. Rep. 21(2):395-401(2009)
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