- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
NF kappa B Antibody (C-term S536)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP16870b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP16870b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
NF kappa B Antibody (C-term S536) - Product info | |
| Application | WB
|
| Primary Accession | Q04206 |
| Other Accession | Q04207, NP_001138610.1, NP_068810.3 |
| Reactivity | Human |
| Predicted | Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 60219 Da |
NF kappa B Antibody (C-term S536) - Additional info | |
| Gene ID 5970 | |
| Other Names RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 | |
| Target/Specificity This NF kappa B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 514-542 amino acids from the C-terminal region of human NF kappa B. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions NF kappa B Antibody (C-term S536) is for research use only and not for use in diagnostic or therapeutic procedures. | |
NF kappa B Antibody (C-term S536) - Protein Information | |
| Name RELA | |
| Synonyms NFKB3 | |
| Function NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1 | |
| Cellular Location Nucleus. Cytoplasm. Note=Colocalized with DDX1 in the nucleus upon TNF-alpha induction (By similarity) Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation | |
NF kappa B Antibody (C-term S536) - Related products
AP16870b: NF kappa B Antibody (C-term S536)
AP3176a: Phospho-NFKBp65-S536 Antibody
AP3178a: Phospho-NFKB-S536 Antibody
AP3717a: Phospho-NF kappa B-S276 Antibody
AP3749a: Phospho-NF kappa B-S536 Antibody
AP6826a: RELA Antibody (N-term)
AP6826c: RELA Antibody (Center)
DC03759: Human RELA cDNA Clone
LY10116a: RELA Over-expression Lysate
BP3176a: Phospho-NFKBp65-S536 Antibody Blocking Peptide
BP3178a: Phospho-NFKB-pS536 Antibody Blocking Peptide
BP3717a: Phospho-NF kappa B-S276 Antibody Blocking Peptide
BP6826a: RELA Antibody (N-term) Blocking Peptide
BP6826c: RELA Antibody (Center) Blocking Peptide
BP9125c: NF kappa B-S276 Antibody Blocking Peptide
BP9455a: NF kappa B-S276 Blocking Peptide
AT3616a: RELA Antibody (monoclonal) (M01)
AJ1537a: NF-kappaB Antibody (p65 subunit)
NF kappa B Antibody (C-term S536) - Application data
-
NF kappa B Antibody (C-term S536) (Cat. #AP16870b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NF kappa B antibody detected the NF kappa B protein (arrow).
NF kappa B Antibody (C-term S536) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
REFERENCES
Pan, W.W., et al. J. Biol. Chem. 285(45):34348-34354(2010) Tago, K., et al. J. Biol. Chem. 285(40):30622-30633(2010) Park, J.S., et al. Oncol. Rep. 24(3):709-714(2010) Yu, Z.H., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 26(7):650-652(2010) Rohwer, N., et al. PLoS ONE 5 (8), E12038 (2010) :
