|Application ||WB, E|
|Other Accession||Q9JME5, NP_004635.2|
|Calculated MW||119059 Da|
|Antigen Region||16-44 aa|
|Other Names||AP-3 complex subunit beta-2, Adaptor protein complex AP-3 subunit beta-2, Adaptor-related protein complex 3 subunit beta-2, Beta-3B-adaptin, Clathrin assembly protein complex 3 beta-2 large chain, Neuron-specific vesicle coat protein beta-NAP, AP3B2|
|Target/Specificity||This AP3B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-44 amino acids from the N-terminal region of human AP3B2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AP3B2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.|
|Cellular Location||Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.|
|Tissue Location||Expression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression)|
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Provided below are standard protocols that you may find useful for product applications.
Adaptor protein-3 (AP3) is a heterotetrameric vesicle-coat protein complex. Some AP3 subunits are ubiquitously expressed, whereas others are expressed exclusively in neurons. The neuron-specific AP3 complex, which includes AP3B2, is thought to serve neuron-specific functions such as neurotransmitter release (Grabner et al., 2006 [PubMed 16788073]).
Nicolas, E., et al. Eur. J. Hum. Genet. 18(10):1107-1113(2010)
Hashimoto, R., et al. Neurosci. Res. 65(1):113-115(2009)
Chen, C., et al. DNA Seq. 18(3):165-168(2007)
Grabner, C.P., et al. Proc. Natl. Acad. Sci. U.S.A. 103(26):10035-10040(2006)
Dubois, T., et al. Biochem. Biophys. Res. Commun. 301(2):502-508(2003)
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