|Application ||WB, E|
|Other Accession||NP_004897.2, NP_690597.1, NP_690596.1|
|Calculated MW||44244 Da|
|Antigen Region||272-301 aa|
|Other Names||Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, hFL(2)D, WT1-associated protein, Wilms tumor 1-associating protein, WTAP, KIAA0105|
|Target/Specificity||This WTAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-301 amino acids from the C-terminal region of human WTAP.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||WTAP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).|
|Cellular Location||Nucleus, nucleoplasm. Nucleus speckle|
|Tissue Location||Ubiquitously expressed.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform.
Su, J., et al. Diabetes Res. Clin. Pract. 87(2):167-175(2010)
Small, T.W., et al. J. Biol. Chem. 284(37):24684-24695(2009)
Benyamin, B., et al. Am. J. Hum. Genet. 84(1):60-65(2009)
Zhong, S., et al. Plant Cell 20(5):1278-1288(2008)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
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