|Application ||WB, E|
|Other Accession||NP_001005369.1, NP_002444.2|
|Calculated MW||81317 Da|
|Antigen Region||138-167 aa|
|Other Names||Translation initiation factor IF-2, mitochondrial, IF-2(Mt), IF-2Mt, IF2(mt), MTIF2|
|Target/Specificity||This MTIF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the N-terminal region of human MTIF2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MTIF2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.|
|Tissue Location||Expressed in all tissues examined. Highest level in skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
Dick, D.M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. (2010) In press :
Reiling, E., et al. Eur. J. Hum. Genet. 17(8):1056-1062(2009)
Miura, E., et al. Plant Cell 19(4):1313-1328(2007)
Bouwmeester, T., et al. Nat. Cell Biol. 6(2):97-105(2004)
Overman, R.G. Jr., et al. Biochim. Biophys. Acta 1628(3):195-205(2003)
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