|Application ||WB, E|
|Other Accession||Q28886, NP_000530.1|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||38893 Da|
|Antigen Region||310-339 aa|
|Other Names||Rhodopsin, Opsin-2, RHO, OPN2|
|Target/Specificity||This RHO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310-339 amino acids from the C-terminal region of human RHO.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RHO Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.|
|Cellular Location||Membrane; Multi-pass membrane protein Note=Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia|
|Tissue Location||Rod shaped photoreceptor cells which mediates vision in dim light|
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Provided below are standard protocols that you may find useful for product applications.
Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness.
Clark, G.R., et al. Ophthalmology 117(11):2169-2177(2010)
Li, S., et al. Biochem. Biophys. Res. Commun. 401(1):42-47(2010)
Pulagam, L.P., et al. J. Biol. Chem. 285(38):29446-29456(2010)
Audo, I., et al. Arch. Ophthalmol. 128(8):1036-1045(2010)
Audo, I., et al. Invest. Ophthalmol. Vis. Sci. 51(7):3687-3700(2010)
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