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RHO Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RHO Antibody (C-term) AP16977b
    All lanes : Anti-RHO Antibody (C-term) at 1:2000 dilution Lane 1: mouse eyeball lysate Lane 2: rat eyeball lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RHO Antibody (C-term) AP16977b
    RHO Antibody (C-term) (Cat. #AP16977b) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RHO antibody detected the RHO protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08100
Other Accession Q28886, NP_000530.1
Reactivity Human, Mouse, Rat
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38893 Da
Antigen Region 310-339 aa
Additional Information
Gene ID 6010
Other Names Rhodopsin, Opsin-2, RHO, OPN2
Target/Specificity This RHO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310-339 amino acids from the C-terminal region of human RHO.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRHO Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RHO
Synonyms OPN2
Function Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Cellular Location Membrane; Multi-pass membrane protein Note=Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia
Tissue Location Rod shaped photoreceptor cells which mediates vision in dim light
Research Areas
Citations (0)

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Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness.


Clark, G.R., et al. Ophthalmology 117(11):2169-2177(2010)
Li, S., et al. Biochem. Biophys. Res. Commun. 401(1):42-47(2010)
Pulagam, L.P., et al. J. Biol. Chem. 285(38):29446-29456(2010)
Audo, I., et al. Arch. Ophthalmol. 128(8):1036-1045(2010)
Audo, I., et al. Invest. Ophthalmol. Vis. Sci. 51(7):3687-3700(2010)

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Cat# AP16977b
(40 western blots)
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