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CARM1 Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP16980c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP16980c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
CARM1 Antibody (Center) - Product info | |
| Application | WB
|
| Primary Accession | Q86X55 |
| Other Accession | Q4AE70, Q9WVG6, NP_954592.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 65854 Da |
CARM1 Antibody (Center) - Additional info | |
| Gene ID 10498 | |
| Other Names CARM1; PRMT4; Histone-arginine methyltransferase CARM1; Coactivator-associated arginine methyltransferase 1; Protein arginine N-methyltransferase 4 | |
| Target/Specificity This CARM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human CARM1. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions CARM1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
CARM1 Antibody (Center) - Protein Information | |
| Name CARM1 | |
| Synonyms PRMT4 | |
| Function Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg- 2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA- stabilizing properties and the half-life of their target mRNAs | |
| Cellular Location Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane | |
| Tissue Location Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia | |
CARM1 Antibody (Center) - Related products
CARM1 Antibody (Center) - Application data
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CARM1 Antibody (Center) (Cat. #AP16980c) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CARM1 antibody detected the CARM1 protein (arrow).
CARM1 Antibody (Center) - Research Areas
AntibodiesBiological ProcessSignal TransductionPost-translational Protein ModificationProtein Modification ProcessBiopolymer ModificationMetabolic ProcessPrimary Metabolic ProcessProtein Metabolic ProcessCellular Protein Metabolic ProcessCellular Metabolic ProcessCellular ProcessMacromolecule Metabolic ProcessIntracellular Signaling CascadeCellular Macromolecule Metabolic ProcessInterspecies Interaction Between OrganismsRegulation Of Metabolic ProcessCellular Component OrganizationRegulation Of Cellular Metabolic ProcessMulti-organism ProcessBiological RegulationRegulation Of Macromolecule Metabolic ProcessCovalent Chromatin ModificationOrganelle OrganizationRegulation Of Primary Metabolic ProcessOne-carbon Metabolic ProcessHistone ModificationRegulation Of Biological ProcessBiosynthetic ProcessCellular Biosynthetic ProcessIntracellular Receptor-mediated Signaling PathwayRegulation Of Cellular ProcessNitrogen Compound Metabolic Process
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BACKGROUND
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).
REFERENCES
Gao, X., et al. J. Cell. Biochem. 110(1):162-170(2010) Carascossa, S., et al. Genes Dev. 24(7):708-719(2010) Kim, Y.R., et al. BMC Cancer 10, 197 (2010) : Ito, T., et al. BMC Dev. Biol. 9, 47 (2009) : Haiman, C.A., et al. BMC Cancer 9, 43 (2009) :
