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HEYL Antibody (N-term)
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
United StatesOrdering Information
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(40 western blots)
$ 265.00email & fax$ 251.75online
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(8 western blots)
$ 95.00email & fax$ 90.25online
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HEYL Antibody (N-term) - Product info
|Application ||WB, IF|
|Other Accession||Q9DBX7, Q2NL18, NP_055386.1|
|Calculated MW||35087 Da|
HEYL Antibody (N-term) - Additional info
|Gene ID 26508|
HEYL; BHLHB33; HRT3; Hairy/enhancer-of-split related with YRPW motif-like protein; Class B basic helix-loop-helix protein 33; Hairy-related transcription factor 3
This HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human HEYL.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
HEYL Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
HEYL Antibody (N-term) - Protein Information
|Synonyms BHLHB33, HRT3|
Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Nucleus (By similarity).
HEYL Antibody (N-term) - Related products
HEYL Antibody (N-term) - Research Areas
HEYL Antibody (N-term) (Cat. #AP16997a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow).
Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term)(Cat#AP16997a).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq].
Kokubo, H., et al. Trends Cardiovasc. Med. 15(5):190-194(2005)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Iso, T., et al. J. Cell. Physiol. 194(3):237-255(2003)
Nakagawa, O., et al. Proc. Natl. Acad. Sci. U.S.A. 97(25):13655-13660(2000)
Firulli, B.A., et al. J. Biol. Chem. 275(43):33567-33573(2000)