Catalog #
  • ---All---
  • Uniprot Id
  • Catalog #

HEYL Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Ordering Information
Catalog # SizeAvailability Price  
AP16997A 400 µl
(40 western blots)
In Stock
$ 265.00email & fax$ 251.75online
Add to cart
AP16997A-ev 80 µl
(8 western blots)
In Stock
$ 95.00email & fax$ 90.25online
Add to cart
  • Specification
  • Citations : 0
  • Reviews
  • Protocols
  • Background

HEYL Antibody (N-term) - Product Information

Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF
Primary AccessionQ9NQ87
Other AccessionQ9DBX7, Q2NL18, NP_055386.1
ReactivityHuman
PredictedBovine, Mouse
Immunity Source (Host) Rabbit
Isotype Rabbit Ig
Calculated MW 35087 Da
Antigen Region 26-54

HEYL Antibody (N-term) - Additional Information

Gene ID 26508
Other Names
HEYL; BHLHB33; HRT3; Hairy/enhancer-of-split related with YRPW motif-like protein; Class B basic helix-loop-helix protein 33; Hairy-related transcription factor 3
Target/Specificity
This HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human HEYL.
Dilution
WB~~1:1000
IF~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
HEYL Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

HEYL Antibody (N-term) - Protein Information

Name HEYL
Synonyms BHLHB33, HRT3
Function
Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. {ECO:0000250, ECO:0000269|PubMed:15485867}.
Cellular Location
Nucleus {ECO:0000255|PROSITE- ProRule:PRU00380, ECO:0000255|PROSITE-ProRule:PRU00981}

HEYL Antibody (N-term) - Related Products

BP16997a: HEYL Antibody (N-term) Blocking Peptide

AT2359a: HEYL Antibody (monoclonal) (M02)

AT2360a: HEYL Antibody (monoclonal) (M07)

HEYL Antibody (N-term) - Research Areas

Application Data

  • WB - HEYL Antibody (N-term) AP16997A

    HEYL Antibody (N-term) (Cat. #AP16997a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow).

  • IF - HEYL Antibody (N-term) AP16997A

    Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term)(Cat#AP16997a).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.

  • WB - HEYL Antibody (N-term) AP16997A
  • IF - HEYL Antibody (N-term) AP16997A

ELITE™ Custom Antibody Services

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Thank you for your support.


Submit

Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq].

REFERENCES

Kokubo, H., et al. Trends Cardiovasc. Med. 15(5):190-194(2005)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Iso, T., et al. J. Cell. Physiol. 194(3):237-255(2003)
Nakagawa, O., et al. Proc. Natl. Acad. Sci. U.S.A. 97(25):13655-13660(2000)
Firulli, B.A., et al. J. Biol. Chem. 275(43):33567-33573(2000)