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>   home   >   Products   >   Primary Antibodies   >   HEYL Antibody (N-term)   

HEYL Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HEYL Antibody (N-term) AP16997A
    HEYL Antibody (N-term) (Cat. #AP16997a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow).
  • IF - HEYL Antibody (N-term) AP16997A
    Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term)(Cat#AP16997a).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9NQ87
Other Accession Q9DBX7, Q2NL18, NP_055386.1
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35087 Da
Antigen Region 26-54 aa
Additional Information
Gene ID 26508
Other Names Hairy/enhancer-of-split related with YRPW motif-like protein, hHeyL, Class B basic helix-loop-helix protein 33, bHLHb33, Hairy-related transcription factor 3, HRT-3, hHRT3, HEYL, BHLHB33, HRT3
Target/Specificity This HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human HEYL.
Dilution WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Protein Information
Name HEYL
Synonyms BHLHB33, HRT3
Function Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00380, ECO:0000255|PROSITE-ProRule:PRU00981}
Citations (0)

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Background

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq].

References

Kokubo, H., et al. Trends Cardiovasc. Med. 15(5):190-194(2005)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Iso, T., et al. J. Cell. Physiol. 194(3):237-255(2003)
Nakagawa, O., et al. Proc. Natl. Acad. Sci. U.S.A. 97(25):13655-13660(2000)
Firulli, B.A., et al. J. Biol. Chem. 275(43):33567-33573(2000)

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$ 265.00
$ 95.00
Cat# AP16997A
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(40 western blots)
Availability: In Stock
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