RPN1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | P04843 |
Other Accession | P07153, Q9GMB0, Q4R4T0, NP_002941.1 |
Reactivity | Human |
Predicted | Monkey, Pig, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 68569 Da |
Antigen Region | 559-587 aa |
Gene ID | 6184 |
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Other Names | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1, RPN1 |
Target/Specificity | This RPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 559-587 amino acids from the C-terminal region of human RPN1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RPN1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RPN1 (HGNC:10381) |
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Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. |
Cellular Location | Endoplasmic reticulum {ECO:0000250|UniProtKB:E2RQ08, ECO:0000250|UniProtKB:Q9GMB0} Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
Tissue Location | Expressed in all tissues tested. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
References
Ruiz-Canada, C., et al. Cell 136(2):272-283(2009)
Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
Wilson, C.M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(28):9534-9539(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
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