|Application ||WB, E|
|Other Accession||Q99PW4, NP_291028.3|
|Calculated MW||28160 Da|
|Antigen Region||221-250 aa|
|Other Names||TP53-regulating kinase, Atypical serine/threonine protein kinase TP53RK, EKC/KEOPS complex subunit TP53RK, 36--, Nori-2, p53-related protein kinase, TP53RK, C20orf64, PRPK|
|Target/Specificity||This TP53RK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human TP53RK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TP53RK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:27903914). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:27903914). TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation (PubMed:11546806).|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Highly expressed in testis. Weakly expressed in heart kidney and spleen.|
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Provided below are standard protocols that you may find useful for product applications.
Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Facchin, S., et al. Cell. Mol. Life Sci. 64 (19-20), 2680-2689 (2007) :
Abe, Y., et al. Biochem. Biophys. Res. Commun. 344(1):377-385(2006)
Facchin, S., et al. FEBS Lett. 549 (1-3), 63-66 (2003) :
Miyoshi, A., et al. Biochem. Biophys. Res. Commun. 303(2):399-405(2003)
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