|Application ||WB, E|
|Other Accession||Q99PW4, NP_291028.3|
|Calculated MW||28160 Da|
|Antigen Region||221-250 aa|
|Other Names||TP53-regulating kinase, Atypical serine/threonine protein kinase TP53RK, EKC/KEOPS complex subunit TP53RK, 36--, Nori-2, p53-related protein kinase, TP53RK, C20orf64, PRPK|
|Target/Specificity||This TP53RK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human TP53RK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TP53RK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.|
|Tissue Location||Highly expressed in testis. Weakly expressed in heart kidney and spleen|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Facchin, S., et al. Cell. Mol. Life Sci. 64 (19-20), 2680-2689 (2007) :
Abe, Y., et al. Biochem. Biophys. Res. Commun. 344(1):377-385(2006)
Facchin, S., et al. FEBS Lett. 549 (1-3), 63-66 (2003) :
Miyoshi, A., et al. Biochem. Biophys. Res. Commun. 303(2):399-405(2003)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.