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CD1D Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CD1D Antibody (Center) AP17014c
    CD1D Antibody (Center) (Cat. #AP17014c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CD1D antibody detected the CD1D protein (arrow).
    detail
  • WB - CD1D Antibody (Center) AP17014c
    Western blot analysis of CD1D (arrow) using rabbit polyclonal CD1D Antibody (Center) (Cat. #AP17014c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD1D gene.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P15813
Other Accession NP_001757.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 37717 Da
Antigen Region 177-206 aa
Additional Information
Gene ID 912
Other Names Antigen-presenting glycoprotein CD1d, R3G1, CD1d, CD1D
Target/Specificity This CD1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the Central region of human CD1D.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD1D Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD1D
Function Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T- cells.
Cellular Location Cell membrane; Single-pass type I membrane protein. Basolateral cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.
Tissue Location Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues
Research Areas
Citations (0)
citation

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Background

This gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail.

References

Miura, S., et al. J. Virol. 84(22):11614-11623(2010)
Moll, M., et al. Blood 116(11):1876-1884(2010)
Liu, J., et al. J. Immunol. 184(9):4973-4981(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Brandl, C., et al. PLoS ONE 5 (5), E10800 (2010) :

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$ 365.00
$ 140.00
Cat# AP17014c
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